Microecological examination of humans using the method of gas chromatography-mass spectrometry (GC-MS)
The existing method of microbiological examination of the patient in clinical laboratories is limited to the analysis of only a dozen kinds of aerobic microorganisms among enterobacteria, aerobic cocci and pseudomonades for many reasons.
Many clinically important microbes among aerobic actinobacteria, all anaerobes and other difficult to cultivate microorganisms are involuntarily ignored. The number of unaccounted microorganisms in the examination of each patient is hundreds of species, because, as it has been known for a long time, the human body and the environment contains over five hundred species, capable of causing an infectious process or inflammation. Using the information capacity of the Internet, it is not difficult to show that each microbe is potentially pathogenic.
Lactobacilli and bifidobacteria, characterized as absolutely useful microbes, are agents of many inflammatory processes, including septicemic conditions and endocarditis. Nowadays, there is no doubt that infections and inflammations are not monoetiological. Sooner or later we discover that they include a group of microorganisms united in genetically and trophically organized communities, the so-called biofilms.
The human body itself is the main source of microbes, first of all, anaerobes. Anaerobes are dominants of the human microbiota. Their habitats are dense mucopeptides of the mucous membranes of intestine, respiratory tract, urogenital tract and skin compartments without direct access of oxygen. Aerobes are not typical for such habitats of microorganisms.
Modern scientific ideas about the human microecology say that aerobes are secondary in quantitative (and functional) aspect in human organs in health and in disease. Sources of infection, except for especially dangerous ones, are mostly concentrated within a human being, and not in the environment. The share of anaerobes prevails significantly over aerobes and it is high time to take into account in the practice of routine analyses of clinical microbiology laboratories.
In this situation, aerobes, which are the main subject of work of clinical laboratories, are just biological markers of the main infection caused by anaerobes.
Thus, the existing practice of clinical bacteriological studies has little informative value and doubtful value for the treatment of diseases of microbial etiology. A way out of this situation is either expansion and deepening of the procedure for studies by cultural biochemical method with mandatory inclusion of anaerobes and actinobacteria in the permanent practice with improvement of sampling techniques or implementation of new technologies of microbiological analysis without defects due to the need to obtain the biomass of living microorganisms in artificial conditions.
In this regard, the method of gas chromatography-mass spectrometry of microbial markers (GC-MSmm) is very promising.
It is based on high-precision determination of the presence of molecular signs of microorganisms from among their cellular lipids – higher fatty acids, aldehydes, alcohols and sterols in the analyzed sample.
The determination is performed using a highly sensitive and selective method of gas chromatography-mass spectrometry (GC-MS), allowing to measure over a hundred microbial markers at the same time directly in the analyzed material – blood, urine, bioptates, punctates, sputum, and other biological fluids and tissues without preliminary inoculation on culture media or use of test biochemical materials.
There is an automatic analysis algorithm using standard GC-MS programs, allowing one to determine the concentration of over 50 microorganisms in the material three hours after its receipt in the laboratory. The method is confirmed by patents for inventions and has a license for use as a new medical technology.
Many years of experience of application of the method of microbial markers in the clinics showed its practical value in diagnosis and treatment of simple and complex pathologies. It is stipulated by the receipt of an unprecedentedly large amount of information about the bacteria in inflammatory processes and in dysbiosis, especially anaerobes, aerobes that are not cultivated in clinical laboratories, as well as actinobacteria, yeast and microscopic fungi.
The novelty of the analytical procedure and the novelty and volume of information ensure complete understanding of the microbial etiology of the disease of each one of the thousands of examined patients without exception. On the other hand, it requires from the doctors fundamentally new approaches in the treatment of patients on the basis of changed ideas about the microbial ecology of humans in health and disease.
The method has become highly-demanded, because it is informative, express and cost-effective. And most importantly, it gives a tangible advantage in the treatment of chronic diseases, overcoming septicemic conditions and identification of causes of fevers of unknown etiology, as well as the essence of disorders of the general microecological homeostasis of the human body.
Dear colleagues!
We encourage you to use the method of CMS actively in your medical practice. Hundreds of specialists have already evaluated this method and use it as a reliable diagnostic tool.
Geography of centers where the method is used and you can get advice on the results of analyzes:
Austria, United Kingdom, Hungary, Kazakhstan, Latvia, Russia, Ukraine, Estonia.
Today, the GC-MS method has proven itself among specialists in preventive and personalized medicine, gynecologists, reproductologists, urologists, dermatologists, gastroenterologists, space biology and medicine specialists, both as an alternative and when combined with traditional examination methods.
The method has been tested for many years and is effectively used in many medical institutions.
Here are some of them:
- Dr Leschen Functional Medicine, Stirling, Scotland, UK
g.uk/nutritionists/elena-leschenhttps://www.nutritionist-resource.or
- Kovalenko Clinic, Budapest, Sopron, Hungary
https://kovalenkoklinika.hu/en/
- Kovalenko Clinic, Wien, Austria
https://kovalenkoklinika.hu/elerhetoseg/wien/
- Russian Federation State Research Center Institute of Biomedical Problems RAS (IBMP), Moscow, Russia
http://www.istc.int/en/institute/9799
- Nikiforov Russian Center of Emergency and Radiation Medicine, St.-Petersburg, Russia
- “Military Medical Academy named after S. M. Kirov” of the Ministry of Defense of the Russian Federation, St.-Petersburg, Russia
- American Medical Clinic, St.-Petersburg, Russia
- International Medical Center “On Clinic”, Moscow, Russia
- Union Clinics Medical Center is part of the international medical network, as a branch of the Moscow “On Clinic” medical center, St.-Petersburg, Russia
- Verba Mayr Austrian health center, Pushkino, Moscow region, Russia,